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Kembali| NIM (Student Number) | G1F013019 |
|---|---|
| Nama Mahasiswa | TRIANA DEWI |
| Judul Artikel | EFEKTIFITAS FRAKSI ETIL ASETAT DAUN SIRSAK (Annona muricata L.) TERHADAP PENURUNAN KADAR ASAM URAT MENCIT JANTAN Balb/C YANG DIINDUKSI POTASIUM OKSONAT |
| Abstrak | INTISARI Latar Belakang: Fraksi Etil Asetat (FEA) mempunyai kemampuan yang baik dalam menarik senyawa flavonoid. Kandungan flavonoid pada daun sirsak (Annona muricata L.) terbukti dapat menurunkan kadar asam urat dalam darah. Penelitian ini bertujuan untuk mengetahui efektifitas dan pengaruh peningkatan dosis FEA daun sirsak terhadap penurunan kadar asam urat mencit jantan Balb/C yang diinduksi potasium oksonat. Metodologi: Penelitian eksperimental dengan rancangan penelitian yaitu Post Test Only Controlled Group Design. Subyek uji dibagi 5 kelompok yaitu kelompok kontrol negatif (Aquades), kontrol positif (Allopurinol 10 mg/kgBB) dan 3 perlakuan FEA daun sirsak (140, 280, 420 mg/kgBB). Mencit diberi sediaan uji (p.o) selama 7 hari. Pada hari ke 8, diinduksi potasium oksonat 250 mg/KgBB (i.p) 1 jam setelah induksi potasium oksonat diberi sediaan uji (p.o) dan 2 jam setelah induksi potasium oksonat dilakukan pengambilan darah melalui sinus orbitalis. Darah yang didapat dilakukan pengukuran kadar asam urat menggunakan spektrofotometer UV-Vis. Hasil kadar asam urat diuji statistik dengan Kruskal-Wallis dan dilanjutkan dengan uji Mann-Whihney. Hasil Penelitian: Hasil penelitian menunjukkan bahwa FEA daun sirsak dosis 140 mg/kgBB dan 420 mg/kgBB dapat menurunkan kadar asam urat dengan persentase penurunan sebesar 33,772 % dan 39,305 % (p>0,05). Kesimpulan: FEA daun sirsak dosis 140 mg/kgBB dan 420 mg/kgBB mampu menurunkan kadar asam urat pada mencit yang diinduksi potasium oksonat. Kata Kunci: Annona muricata L, Fraksi Etil Asetat, Potasium Oksonat, Kadar Asam Urat. |
| Abstrak (Inggris) | ABSTRACT Background: Ethyl Acetate Fraction (FEA) has a good ability to attract flavonoid compounds. The content of flavonoids in soursop leaves (Annona muricata L.) proven to reduce levels of uric acid in the blood. This study to determine effectiveness and effect of increased dose of FEA soursop leaves to decrease uric acid level of male Balb/C mice induced potassium oxonate. Methodology: Experimental research with research design that is Post Test Only Controlled Group Design. The test subjects were divided into 5 groups: negative control group (Aquadest), positive control (Allopurinol 10 mg/kgBB) and 3 FEA treatment of soursop leaf (140, 280, 420 mg/kgBB). Mice were given test preparation (p.o) for 7 days. On day 8, potassium oxonate 250 mg/KgBB (i.p) induced 1 hour after induction of potassium oxonate was given test preparation (p.o) and 2 hours after potassium oxonate induction was taken by blood orbitalis sinus Blood obtained measurements of uric acid levels using UV-Vis spectrophotometer. The results of uric acid levels were tested statistically with Kruskal-Wallis and followed by the Mann-Whihney test. Result: The results showed that FEA soursop leaves dose 140 mg/kgBB and 420 mg/kgBB can decrease uric acid level with percentage decrease equal to 33,772% and 39,305% (p> 0,05). Conclusion: FEA leaves soursop dose 140 mg/kgBB and 420 mg/kgBB able to reduce uric acid levels in mice induced potassium oxonate. Keywords: Annona muricata L, Ethyl Acetate Fraction, Potassium Oxonate, Uric Acid Levels. |
| Kata Kunci | Kata Kunci: Annona muricata L, Fraksi Etil Asetat, Potasium Oksonat, Kadar Asam Urat. |
| Nama Pembimbing 1 | Dr. Sri Sutji Susilowati, M.Si., Apt |
| Nama Pembimbing 2 | Esti Dyah Utami, M.Sc., Apt |
| Tahun | 2013 |
| Jumlah Halaman | 10 |
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